This eSession course is a four-part series discussing mononuclear cell (MNC) collection procedures on the COBE Spectra system.
Part one, Overview of MNC, covers the factors that may influence blood cell development or hematopoiesis, the types and functions of cells that form the MNC layer, the indication for performing an MNC procedure, and the factors that affect collection efficiency.
Part two, Operational Principles, covers MNC single-stage channel separation, the function of QuickStart, steps to achieve and maintain interface stability, and the factors to consider when optimizing the procedure.
Part three, Troubleshooting MNC, covers actions to take when a patient or donor has a citrate reaction, the effects that increasing the inlet:AC ratio has on the extracorporeal circuit and on the collected product, the potential causes of interface instability, the potential causes of a low CD34+ cell yield.
Part four includes a presentation,Using the White Blood Cell Functionally Closed (FC) Tubing Set, on the COBE Spectra system. This presentation covers the features that make the WBC-FC set a functionally closed system, the situations that cause the set to no longer be functionally closed, how to add ACD-A to the product bag, how to obtain a sample using the sample bulb assembly, and the reasons why it is necessary to reduce the inlet flow during rinseback.
Learning Objectives
Following completion of this course, the participant will be able to:
- Briefly describe what factors may influence blood cell development or hematopoiesis
- Name the types and functions of cells that form the MNC layer
- List two indications for performing an MNC procedure
- Identify three factors affecting collection procedure efficiency
- Describe the COBE Spectra system single-stage channel separation process
- Explain the function of Quick Start
- List the four steps required to achieve and maintain interface stability
- Name three factors to consider when optimizing MNC procedures
- State the first action to take when a patient or donor has a citrate reaction
- Describe the effect that increasing the inlet:AC ratio has on the extracorporeal circuit and on the collected product
- List two potential causes of interface instability
- List two potential causes of a low CD34+ cell yield
Click the links below to register and view the Performing MNC Procedures eSessions Series