This session is made up of two parts: Operational Principles of LRS Turbo Platelet Collection and COBE Spectra System Collection Optimization. Operational Principles of LRS Turbo Platelet Collection covers separation and leukoreduction on the COBE Spectra system, the difference between inlet:AC ratio and AC infusion rate. The importance of accurate donor total blood volume, the effect of donor comfort, yield and concentration, and platelet product quality are also reviewed. The Collection Optimization session covers procedural elements, data entry, procedure results and fluid balance.

Learning Objectives

Following completion of this course, the participant will be able to:

• Describe indications for cellular depletions
• Describe procedural elements
• Describe data entry and procedure results
• Describe fluid balance

This eSession course is a three-part series on therapeutic plasma exchange (TPE) procedures on the COBE Spectra system.

Part one, Overview of TPE, details rational behind TPE, the role of TPE in the treatment of autoimmune diseases and the procedural elements of TPE.

Part two, Operational Principles of TPE, covers the meaning of the values displayed on the end result screen, the percentage disease mediator removed by a 1.0 plasma volume exchange, the correlation between plasma volume exchanged, percentage of disease mediator removed and therapeutic effectiveness of a TPE procedure, the distribution of AC added to the extra corporeal circuit; where the AC is coming from and where it is going, the data entry parameter which has an effect on the RBC/plasma interface, and the factors that determine the fluid balance.

Part three, Troubleshooting TPE, covers actions that must be taken before the procedure can be continued after an alarm condition, reasons for the message "No RBCs detected" and which detector prompts that message, actions that should be taken when a patient has a citrate reaction, risks to the extracorporeal circuit when it is increasing the Inlet: AC ratio, potential causes of the "Plasma line contamination detected" alarm, and lab values that should be checked when performing a plasma exchange procedure with albumin replacement.

Learning Objectives

• Define TPE
• Describe the role of TPE in the treatment of autoimmune diseases
• Explain the meaning of the values displayed on the end result screen
• State the percentage disease mediator removed by a 1.0 plasma volume exchange
• Explain the correlation between plasma volume exchanged, percentage of disease mediator removed and therapeutic effectiveness of a TPE procedure
• Explain the distribution of AC added to the extra corporeal circuit; where the AC is coming from and where it is going
• Identify the data entry parameter which has an effect on the RBC/plasma interface
• List two factors that determine the fluid balance
• State what has to occur before the procedure can be continued after an alarm condition on the COBE Spectra system
• State one reason for the message "No RBCs detected" and which detector prompts that message
• State the first action that should be taken on the COBE Spectra system when a patient has a citrate reaction
• State what the most likely risk to the extracorporeal circuit is when increasing the inlet:AC ratio on the COBE Spectra system, list two potential causes of the "Plasma line contamination detected" alarm on the COBE Spectra system.
• List two lab values that should be checked when performing a plasma exchange procedure with albumin replacement

This eSession course is a four-part series discussing mononuclear cell (MNC) collection procedures on the COBE Spectra system.

Part one, Overview of MNC, covers the factors that may influence blood cell development or hematopoiesis, the types and functions of cells that form the MNC layer, the indication for performing an MNC procedure, and the factors that affect collection efficiency.

Part two, Operational Principles, covers MNC single-stage channel separation, the function of QuickStart, steps to achieve and maintain interface stability, and the factors to consider when optimizing the procedure.

Part three, Troubleshooting MNC, covers actions to take when a patient or donor has a citrate reaction, the effects that increasing the inlet:AC ratio has on the extracorporeal circuit and on the collected product, the potential causes of interface instability, the potential causes of a low CD34+ cell yield.

Part four includes a presentation,Using the White Blood Cell Functionally Closed (FC) Tubing Set, on the COBE Spectra system. This presentation covers the features that make the WBC-FC set a functionally closed system, the situations that cause the set to no longer be functionally closed, how to add ACD-A to the product bag, how to obtain a sample using the sample bulb assembly, and the reasons why it is necessary to reduce the inlet flow during rinseback.

Learning Objectives

Following completion of this course, the participant will be able to:

• Briefly describe what factors may influence blood cell development or hematopoiesis
• Name the types and functions of cells that form the MNC layer
• List two indications for performing an MNC procedure
• Identify three factors affecting collection procedure efficiency
• Describe the COBE Spectra system single-stage channel separation process
• Explain the function of Quick Start
• List the four steps required to achieve and maintain interface stability
• Name three factors to consider when optimizing MNC procedures
• State the first action to take when a patient or donor has a citrate reaction
• Describe the effect that increasing the inlet:AC ratio has on the extracorporeal circuit and on the collected product
• List two potential causes of interface instability
• List two potential causes of a low CD34+ cell yield

This eSession course is a three-part series discussing AutoPBSC collection procedures on the COBE Spectra system.

Part one, Operational Principles of the COBE Spectra System AutoPBSC Collection Procedure, covers the indications for performing a Spectra AutoPBSC procedure, the three phases of collections, the effects of entered data on the calculation of Harvest phase frequency, and the functions of the CCM during the Spectra AutoPBSC procedure.

Part two, Troubleshooting Spectra AutoPBSC, covers the three alarm conditions that place the system in Assisted mode, the actions to take in response to the alarm "CCM Calibration Failure," and the difference between a dark spillover and a light spillover.

Part three of this series is a presentation on the use of Blood Prime on the COBE Spectra system. This presentation covers when to consider performing a Blood Prime, how to perform a Blood Prime and considerations when performing an apheresis procedure using a Blood Prime.

Learning Objectives

Following completion of this course, the participant will be able to:

• State the indications for performing a Spectra AutoPBSC procedure
• Describe the three phases of collection
• Describe how to use Assisted mode
• Explain the effect of entered data on the calculation of Harvest phase frequency
• List the three functions of the CCM during a Spectra AutoPBSC procedure
• Name three factors to consider when optimizing procedures
• List the three alarm conditions that place the operator in Assisted mode
• Explain the action in response to the alarm “CCM Calibration Failure”
• Describe two circumstances when Assisted mode may be indicated
• State the difference between a dark spillover and a light spillover

This course covers all processing procedures on the Elutra^® Cell Separation System and the COBE^® 2991 Cell Processor, and bone marrow processing procedures using the COBE Spectra system.

Part one, Cellular Elutriation using the Elutra System, provides an overview of elutriation, basic operational principles of the Elutra system, starting product conditions required to achieve a successful monocyte enrichment, data entry considerations, and helpful hints for Elutra system users.

Part two, COBE 2991 Cell Processor Overview, provides a brief description of the operational principles, validated applications and the use of the LED program board.

Part three, Bone Marrow Processing using the COBE Spectra system, details the factors that must be considered prior to starting bone marrow processing procedure, the default values and procedure parameters for bone marrow processing procedures, the minimum RBC volumes required to perform a bone marrow processing procedures, and the process for determining when to process more than the default inlet volume.

Learning Objectives

Following completion of this course, the participant will be able to:

• Describe the two cell characteristics that make the separation of cell populations possible during elutriation
• List three choices that must be made prior to loading the disposable tubing set
• Identify three calculations the Elutra system makes using the starting cell product data
• State two effects of inaccurate starting cell product data on an elutriation procedure
• List the four starting cell product conditions required to achieve a monocyte collection with an average purity of 80% and an average recovery of 60% using the Elutra system
• Name four actions to avoid when performing an elutriation procedure
• List the three operations that constitute a wash cycle using the COBE 2991 Cell Processor
• State the three LED program board modes using the COBE 2991 Cell Processor
• Explain STOP SELECT and define CALL, R.C. and P.C. when using the COBE 2991 Cell Processor
• Describe how to program a Hold at the start of the Agitate Wash-In operation of the COBE 2991 Cell Processor
• State the purpose of bone marrow processing
• Identify the disposable tubing sets required for bone marrow processing
• List three factors that must be considered prior to starting a bone marrow processing procedure
• Name the default values and procedure parameters for bone marrow processing procedures and identify one factor that determines them
• State the minimum RBC volume required to perform a bone marrow processing procedure
• Describe how to determine when to process more than the default inlet volume processed

Learning Objectives

Following completion of this course, the participant will be able to:

• List four features that make the WBC-FC set a functionally closed system
• Identify three situations that cause the set to no longer be functionally closed
• Describe how to add ACD-A to the product bag
• Explain how to obtain a sample using the sample bulb assembly
• State why it is necessary to reduce the inlet flow during Rinseback