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Quantum® Cell Expansion System

Product Confidence. Process Scalability.

The Quantum system is an automated cell culture platform that can help simplify the open, labor-intensive manual tasks associated with flask-based culture, helping improve the reproducibility and scalability of your cell culture process to meet your overall manufacturing requirements.

Browse our materials library below to find technical resources relevant to your cell type or area of interest.

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  • Publications
  • Customer Testimonials/Press
    • Applications (28)

      The Quantum Cell Expansion System, originally designed for cell expansion, has been thriving in a variety of other applications. Its versatility and user-friendly platform have opened up new windows of usage, and its limitations are yet to be found.

      Show All

      • Cell Expansion -- Adherent (27)

        • new GMP-Compliant Expansion of Clinical-Grade hMSCs Using a Closed Hollow-Fiber Bioreactor

          • Human mesenchymal stromal/stem cells (hMSCs) have emerged as attractive candidates for cell therapy, tissue repair and tissue engineering. This abstract describes a method for good manufacturing practice (GMP)-compliant expansion of hMSCs from bone marrow aspirate using the Quantum® Cell Expansion System. The method includes protocols for preparation of media, setup of the Quantum system, coating of the hollow-fiber bioreactor, as well as the loading, feeding and harvesting of cells.

            View

        • new Process Automation in Manufacturing of Mesenchymal Stromal Cells

          • This article summarizes the labor-intensive nature of current mesenchymal stem cell (MSC) production and increasing demand for devices designed specifically to automate MSC production. As therapeutic processes involving MSCs move from early- to late-stage clinical trials, an increased need is also arising for both scale-up and scale-out manufacturing. The Quantum® Cell Expansion System is a hollow-fiber continuous perfusion device designed specifically for growing multiple adherent cell types and suspension cells in a functionally closed environment.

            View

        • new Redefining the Role of Blood Establishments as Raw Material Suppliers, Manufacturers and Distributors for New Cell Therapies: The Blood Systems Experience

          • This article discusses the unique skills and expertise, as well as challenges and considerations, of blood establishments that may pursue market activities extending beyond those typically associated with blood components. Such activities include autologous and allogeneic therapies related to regenerative therapies and personalized medicine, donor testing, patient testing, centralized transfusion services, bone marrow processing to support transplants and other related products and services.

            View

        • Automated Expansion of Human Mesenchymal Stem Cells (MSCs) From Whole Bone Marrow and Precultured Cells Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole bone marrow and precultured cells on the Quantum system utilizing fibronectin or cryoprecipitate as an adhesion promoter.

            View

        • Expansion of Human Mesenchymal Stem Cells (MSCs) in the Quantum System Using Human Platelet Lysate (hPL) as a Cell Culture Media Supplement

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs using human platelet lysate as a cell culture media supplement in conjunction with a cryoprecipitate-coated bioreactor.

            View

        • Automated Ex Vivo Expansion of Human Mesenchymal Stem Cells (MSCs) from Pre-Selected Cells Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole unprocessed bone marrow, eliminating the need for density separation of the bone marrow aspirate.

            View

        • Automated Ex Vivo Expansion of Human Mesenchymal Stem Cells (MSCs) from Unprocessed Bone Marrow Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole unprocessed bone marrow, eliminating the need for density separation of the bone marrow aspirate.

            View

        • Mesenchymal Stem Cells (MSC) Genetic Stability in the Quantum Cell Expansion System

          • This poster explains the assays and results used by Terumo BCT to determine the genetic stability of MSCs produced in the Quantum system. The results conclude that the Quantum system produces cell quality and genetic stability comparable to traditional flasks and meets the Stem Cell Transplantation International Society of Cell Therapy Guidelines for MSC phenotype.

            View

        • Culture of Normal Human Dermal Fibroblast (NHDF) Cells in a Functionally Closed Automated Cell Expansion System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated, scalable expansion method to reproducibly grow NHDFs to yield clinically relevant doses. Additionally, fibronectin and cryoprecipitate are both shown to be feasible coating reagents that can be used to coat the bioreactor prior to expanding NHDFs.

            View

        • Quality Control During Manufacture of a Stem Cell Therapeutic

          • This article from the March 2012 issue of BioProcess International outlines how three research and development scientists from ReGenesys use extensive quality control (QC) to ensure the identity, quality and safety of their therapeutic stem cell product. The paper compares their QC results between cells expanded in the Quantum system to cells obtained using conventional flask-based culture.

            View

        • Using Multiple Freeze-Thawed Cryoprecipitate (CPPT) as a Coating Reagent

          • The results of this study presented at the International Society for Stem Cell Research (ISSCR) in 2013 show that CPPT that has undergone two freeze-thaw events can be used to coat the Quantum system hollow-fiber membrane and produce cell yields that are comparable to CPPT that was only thawed once. These results were demonstrated using: bone marrow-derived mesenchymal stem cells, adipose-derived mesenchymal stem cells and normal human dermal fibroblasts. The use of multiple freeze-thawed CPPT coating offers a more cost-effective coating mechanism.

            View

        • Glucose and Lactate Measurements as a Predictor of the Human Mesenchymal Stem Cell (MSC) Number in the Quantum System

          • This paper describes the use of glucose and lactate measurements for indirect observations of cell expansion within the Quantum system. The results provide a heuristic for determining when the expansion inlet rate of new media should be increased and a means for approximately determining when a desired number of cells can be harvested.

            View

        • Expansion of Adult Bone Marrow-Derived Mesenchymal Stem Cells (BMMSCs) in the Quantum System Under Hypoxic and Normoxic Conditions

          • The results of this study presented at the International Society for Stem Cell Research (ISSCR) in 2013 demonstrate that clinical-scale yields of MSCs may be expanded on the Quantum system under both normoxic and hypoxic conditions. There was no significant difference in cell yield, viability, phenotypic, morphological or differentiation potential observed between cells cultured under either condition.

            View

        • Process Optimization for Expansion of Bone Marrow-Derived Mesenchymal Stem Cells (MSCs)

          • This poster, presented at the International Society for Cellular Therapy (ISCT) in 2011, illustrates the diverse functionality and process control of the Quantum system. An optimized process has many benefits including limiting the media used, thus lowering costs, increasing the cell yield and creating a more uniform cell harvest.

            View

        • Quantitative Tri-Lineage Differentiation Assays for Mesenchymal Stem Cells (MSCs) Grown in the Quantum Cell Expansion System

          • This poster, presented at the International Society for Cellular Therapy (ISCT) in 2011, reports on quantitative assays for MSC adipocyte, osteocyte and chondrocyte differentiation for MSCs grown in the Quantum system. These assays address an unmet need in the MSC field for quantitative differentiation assays eliminating user-to-user subjective assessments and, more importantly, allowing for lab-to-lab quantitative comparisons.

            View

        • Manufacture of Bone Marrow Stromal Cells (BMSCs) Using a Novel Closed System Maintains Cell Characteristics Consistent With Flask-Based Methods

          • This poster, authored by the NIH, describes three major findings. First, cell yield was in all test cases higher when cells were grown in the Quantum system compared to Cell Factories. Second, BMSCs grown in the Quantum system have similar phenotypes compared to BMSCs grown in Cell Factories. Lastly, BMSCs from both the Quantum system and Cell Factories are able to differentiate into adipocytes. In conclusion, growth in the Quantum system does not alter the differentiation potential of the subset of stem cells within the BMSC population.

            View

        • The Quantum Cell Expansion System’s Valuable Role in Cell Production Requirements for ReGenesys

          • This case study outlines the benefits experienced at ReGenesys by using the Quantum system. Specifically this case study outlines a ten-fold reduction in labor and 40 percent cost savings.

            View

        • Managing Contamination Risk While Maintaining Quality in Cell-Therapy Manufacturing

          • With an increasing number of cell therapies becoming available for patient use, the need for controlled and consistent manufacturing and delivery of cell products is increasingly important. The Quantum system's closed cell culture process not only offers control and consistency, but may also relieve labor demands.

            View

        • Exploring Good Manufacturing Practice (GMP) Production of Lentiviral Vectors

          • This poster describes the successful large-scale production of clinical-grade lentiviral vector using the Quantum Cell Expansion System at the University of California (UC) Davis Institute for Regenerative Cures.

            View

        • Successful Culture of Human Adipose-Derived Mesenchymal Stromal Cells (ASCs) in a Functionally Closed Automated Cell Expansion System

          • This white paper demonstrates that robust ASC ex vivo expansion is possible using the Quantum system to reach clinically relevant cell yields starting from precultured ASCs. The study compared the doubling times of Quantum system-expanded ASCs with ASCs grown in T25 control flasks as well as characterization of ASCs by trilineage differentiation, morphology and phenotype.

            View

        • Efficient Manufacturing of Therapeutic Mesenchymal Stromal Cells (MSCs) With the Use of the Quantum Cell Expansion System

          • This article was authored by Patrick Hanley of Children's National Medical Center (formerly of Baylor College of Medicine) and published in the August 2014 edition of Cytotherapy. It evaluates the Quantum system for the cost-effective, safe and reproducible expansion of large numbers of MSCs from unprocessed bone marrow compared with a flask-based method currently used in clinical trials. The results show that the Quantum system was able to generate the target cell number of 2.0 × 108 cells in an average of nine fewer days and in half the number of passages required during flask-based expansion.

            View

        • Expanding Human Mesenchymal Stem Cells (MSCs) to Therapeutic Doses Using Human Platelet Lysate (hPL) in the Quantum Cell Expansion System

          • The aim of this study was to evaluate the flexibility of the Quantum system when users require different combinations of adherence promoters and media supplements. The results demonstrate that the Quantum system can be used effectively to culture MSCs using exclusively human-derived reagents.

            View

        • CD146 Expression as a Biomarker for hMSC Potency in Normal Cells Expanded With the Quantum System

          • The Terumo BCT Innovation and Development (I&D) team has identified the CD146 surface biomarker expression by flow cytometry as an early indicator of hMSC differentiation potency at the time of harvest from the Quantum system. A poster has been developed to illustrate the study and its findings.

            View

        • Culture of Normal Human Dermal Fibroblast (NHDF) Cells in a Functionally Closed Automated Cell Expansion System

          • This scientific poster demonstrates how the Quantum system provides a functionally closed, automated, scalable expansion method to reproducibly grow NHDFs to yield clinically relevant doses. Additionally, fibronectin and cryoprecipitate are both shown to be feasible coating reagents that can be used to coat the bioreactor prior to expanding NHDFs.

            View

        • Genetic Stability of Bone Marrow-Derived Human Mesenchymal Stromal Cells in the Quantum System1

          • In this study, published online in the journal Cytotherapy, a rigorous battery of assays was used to determine the genetic stability of BM-hMSCs selected and produced with the Quantum system. hMSC bone marrow aspirates, obtained from three approved donors were expanded in parallel using T225 culture flasks and the Quantum system. The study concluded that Quantum system-produced BM-hMSCs are of comparable quality and demonstrate analogous genetic stability to BM-hMSCs cultured on tissue culture polystyrene substrates.

            View

        • Shear Stress Conditions in the Quantum Cell Expansion System

          • This scientific poster compares the shear stress experienced by populations of cells in the Quantum system during cell expansion and harvest with the shear stress created in flask environments. The findings show shear stress created in tissue culture flasks during a harvest procedure is significantly higher than the shear stress created in the hollow-fiber bioreactor of the Quantum system during harvest.

            View

      • Cell Expansion -- Suspension (2)

        • new WHITE PAPER: Expansion of T-Cells in an Automated, Functionally Closed Hollow-Fiber Bioreactor System

          • This white paper demonstrates the expansion of T-cells in the Quantum® Cell Expansion System. Results from a single stimulation event showed that the Quantum system produced 90- to 500-fold expansion of T-cells starting from an apheresis blood collection in as few as 13 days. The enhanced manufacturing process control of an automated and functionally closed system can help reduce the labor and the number of open events involved in T-cell expansion, which may result in more efficient production and scale-out of cellular production.

            View

        • Maturation of Dendritic Cells from CD14+ Monocytes in an Automated Functionally Closed Hollow-Fiber Bioreactor System

          • Presented at the 2014 International Society for Cellular Therapy (ISCT) Annual Meeting, this poster describes the transformation of CD14+ monocytes into functionally mature DCs using the Quantum system. Also demonstrated, is how the enhanced manufacturing process control of an automated and functionally closed system helps reduce the labor and open events involved in DC maturation.

            View

      • Viral Vector Production (2)

        • Large-Scale Production of Lentiviral Vector in a Closed System Hollow-Fiber Bioreactor

          • This article from the 2015 issue of Molecular Therapy—Methods & Clinical Development, published by the American Society of Gene & Cell Therapy, outlines how researchers developed a method employing a hollow-fiber bioreactor traditionally used for cell expansion to generate a large-scale system method for the manufacturing of clinical-grade lentiviral vectors suitable for the generation of induced pluripotent stem cells (iPSCs). Their results demonstrated the growth, transfection and vector-producing capability of 293T producer cells in this system.

            View

        • Exploring Good Manufacturing Practice (GMP) Production of Lentiviral Vectors

          • This poster describes the successful large-scale production of clinical-grade lentiviral vector using the Quantum Cell Expansion System at the University of California (UC) Davis Institute for Regenerative Cures.

            View

      • DC Maturation (1)

        • Maturation of Dendritic Cells from CD14+ Monocytes in an Automated Functionally Closed Hollow-Fiber Bioreactor System

          • Presented at the 2014 International Society for Cellular Therapy (ISCT) Annual Meeting, this poster describes the transformation of CD14+ monocytes into functionally mature DCs using the Quantum system. Also demonstrated, is how the enhanced manufacturing process control of an automated and functionally closed system helps reduce the labor and open events involved in DC maturation.

            View

    • Cell Types (25)

      A variety of cell types have been successfully expanded in the Quantum system since its launch in 2011. The list grows quickly as our Terumo BCT Cell Processing Specialists work directly with our customers on adapting their current protocol into the Quantum system. Optimization experiments are closely monitored and a new and improved manufacturing process is established.

      Show All

      • Mesenchymal Stem Cells (MSCs) – Bone Marrow-Derived MSCs (21)

        • new GMP-Compliant Expansion of Clinical-Grade hMSCs Using a Closed Hollow-Fiber Bioreactor

          • Human mesenchymal stromal/stem cells (hMSCs) have emerged as attractive candidates for cell therapy, tissue repair and tissue engineering. This abstract describes a method for good manufacturing practice (GMP)-compliant expansion of hMSCs from bone marrow aspirate using the Quantum® Cell Expansion System. The method includes protocols for preparation of media, setup of the Quantum system, coating of the hollow-fiber bioreactor, as well as the loading, feeding and harvesting of cells.

            View

        • new Process Automation in Manufacturing of Mesenchymal Stromal Cells

          • This article summarizes the labor-intensive nature of current mesenchymal stem cell (MSC) production and increasing demand for devices designed specifically to automate MSC production. As therapeutic processes involving MSCs move from early- to late-stage clinical trials, an increased need is also arising for both scale-up and scale-out manufacturing. The Quantum® Cell Expansion System is a hollow-fiber continuous perfusion device designed specifically for growing multiple adherent cell types and suspension cells in a functionally closed environment.

            View

        • new Redefining the Role of Blood Establishments as Raw Material Suppliers, Manufacturers and Distributors for New Cell Therapies: The Blood Systems Experience

          • This article discusses the unique skills and expertise, as well as challenges and considerations, of blood establishments that may pursue market activities extending beyond those typically associated with blood components. Such activities include autologous and allogeneic therapies related to regenerative therapies and personalized medicine, donor testing, patient testing, centralized transfusion services, bone marrow processing to support transplants and other related products and services.

            View

        • Automated Expansion of Human Mesenchymal Stem Cells (MSCs) From Whole Bone Marrow and Precultured Cells Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole bone marrow and precultured cells on the Quantum system utilizing fibronectin or cryoprecipitate as an adhesion promoter.

            View

        • Expansion of Human Mesenchymal Stem Cells (MSCs) in the Quantum System Using Human Platelet Lysate (hPL) as a Cell Culture Media Supplement

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs using human platelet lysate as a cell culture media supplement in conjunction with a cryoprecipitate-coated bioreactor.

            View

        • Automated Ex Vivo Expansion of Human Mesenchymal Stem Cells (MSCs) from Pre-Selected Cells Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole unprocessed bone marrow, eliminating the need for density separation of the bone marrow aspirate.

            View

        • Automated Ex Vivo Expansion of Human Mesenchymal Stem Cells (MSCs) from Unprocessed Bone Marrow Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole unprocessed bone marrow, eliminating the need for density separation of the bone marrow aspirate.

            View

        • Human Mesenchymal Stem Cells (MSC) Genetic Stability in the Quantum Cell Expansion System

          • This poster explains the assays and results used by Terumo BCT to determine the genetic stability of MSCs produced in the Quantum system. The results conclude that the Quantum system produces cell quality and genetic stability comparable to traditional flasks and meets the Stem Cell Transplantation International Society of Cell Therapy Guidelines for MSC phenotype.

            View

        • Using Multiple Freeze-Thawed Cryoprecipitate (CPPT) as a Coating Reagent

          • The results of this study presented at the International Society for Stem Cell Research (ISSCR) in 2013 show that CPPT that has undergone two freeze-thaw events can be used to coat the Quantum system hollow-fiber membrane and produce cell yields that are comparable to CPPT that was only thawed once. These results were demonstrated using: bone marrow-derived mesenchymal stem cells, adipose-derived mesenchymal stem cells and normal human dermal fibroblasts. The use of multiple freeze-thawed CPPT coating offers a more cost-effective coating mechanism.

            View

        • Glucose and Lactate Measurements as a Predictor of the Human Mesenchymal Stem Cell (MSC) Number in the Quantum System

          • This paper describes the use of glucose and lactate measurements for indirect observations of cell expansion within the Quantum system. The results provide a heuristic for determining when the expansion inlet rate of new media should be increased and a means for approximately determining when a desired number of cells can be harvested.

            View

        • Expansion of Adult Bone Marrow-Derived Mesenchymal Stem Cells (BMMSCs) in the Quantum System Under Hypoxic and Normoxic Conditions

          • The results of this study presented at the International Society for Stem Cell Research (ISSCR) in 2013 demonstrate that clinical-scale yields of MSCs may be expanded on the Quantum system under both normoxic and hypoxic conditions. There was no significant difference in cell yield, viability, phenotypic, morphological or differentiation potential observed between cells cultured under either condition.

            View

        • Process Optimization for Expansion of Bone Marrow-Derived Mesenchymal Stem Cells (MSCs)

          • This poster, presented at the International Society for Cellular Therapy (ISCT) in 2011, illustrates the diverse functionality and process control of the Quantum system. An optimized process has many benefits including limiting the media used, thus lowering costs, increasing the cell yield and creating a more uniform cell harvest.

            View

        • Quantitative Tri-Lineage Differentiation Assays for Mesenchymal Stem Cells (MSCs) Grown in the Quantum Cell Expansion System

          • This poster, presented at the International Society for Cellular Therapy (ISCT) in 2011, reports on quantitative assays for MSC adipocyte, osteocyte and chondrocyte differentiation for MSCs grown in the Quantum system. These assays address an unmet need in the MSC field for quantitative differentiation assays eliminating user-to-user subjective assessments and, more importantly, allowing for lab-to-lab quantitative comparisons.

            View

        • Manufacture of Bone Marrow Stromal Cells (BMSCs) Using a Novel Closed System Maintains Cell Characteristics Consistent With Flask-Based Methods

          • This poster, authored by the NIH, describes three major findings. First, cell yield was in all test cases higher when cells were grown in the Quantum system compared to Cell Factories. Second, BMSCs grown in the Quantum system have similar phenotypes compared to BMSCs grown in Cell Factories. Lastly, BMSCs from both the Quantum system and Cell Factories are able to differentiate into adipocytes. In conclusion, growth in the Quantum system does not alter the differentiation potential of the subset of stem cells within the BMSC population.

            View

        • Efficient Manufacturing of Therapeutic Mesenchymal Stromal Cells (MSCs) With the Use of the Quantum Cell Expansion System

          • This article was authored by Patrick Hanley of Children's National Medical Center (formerly of Baylor College of Medicine) and published in the August 2014 edition of Cytotherapy. It evaluates the Quantum system for the cost-effective, safe and reproducible expansion of large numbers of MSCs from unprocessed bone marrow compared with a flask-based method currently used in clinical trials. The results show that the Quantum system was able to generate the target cell number of 2.0 × 108 cells in an average of nine fewer days and in half the number of passages required during flask-based expansion.

            View

        • Shear Stress Conditions in the Quantum System

          • This white paper compares the shear stress experienced by populations of cells in the Quantum system during cell expansion and harvest with the shear stress created in flask environments. The findings show shear stress created in tissue culture flasks during a harvest procedure is significantly higher than the shear stress created in the hollow-fiber bioreactor of the Quantum system during harvest.

            View

        • Expanding Human Mesenchymal Stem Cells (MSCs) to Therapeutic Doses Using Human Platelet Lysate (hPL) in the Quantum Cell Expansion System

          • The aim of this study was to evaluate the flexibility of the Quantum system when users require different combinations of adherence promoters and media supplements. The results demonstrate that the Quantum system can be used effectively to culture MSCs using exclusively human-derived reagents.

            View

        • CD146 Expression as a Biomarker for hMSC Potency in Normal Cells Expanded With the Quantum System

          • The Terumo BCT Innovation and Development (I&D) team has identified the CD146 surface biomarker expression by flow cytometry as an early indicator of hMSC differentiation potency at the time of harvest from the Quantum system. A poster has been developed to illustrate the study and its findings.

            View

        • Genetic Stability of Bone Marrow-Derived Human Mesenchymal Stromal Cells in the Quantum System1

          • In this study, published online in the journal Cytotherapy, a rigorous battery of assays was used to determine the genetic stability of BM-hMSCs selected and produced with the Quantum system. hMSC bone marrow aspirates, obtained from three approved donors were expanded in parallel using T225 culture flasks and the Quantum system. The study concluded that Quantum system-produced BM-hMSCs are of comparable quality and demonstrate analogous genetic stability to BM-hMSCs cultured on tissue culture polystyrene substrates.

            View

        • Shear Stress Conditions in the Quantum Cell Expansion System

          • This scientific poster compares the shear stress experienced by populations of cells in the Quantum system during cell expansion and harvest with the shear stress created in flask environments. The findings show shear stress created in tissue culture flasks during a harvest procedure is significantly higher than the shear stress created in the hollow-fiber bioreactor of the Quantum system during harvest.

            View

        • GMP-Compliant Isolation and Expansion of Bone Marrow-Derived MSCs in the Closed, Automated, Quantum Cell Expansion System2

          • This paper investigates the Quantum system as an alternative to conventional tissue flasks for clinical-scale mesenchymal stem cell (MSC) isolation and expansion from bone marrow using platelet lysate. The paper concludes that the Quantum system reliably produces a cellular therapeutic dose in a functionally closed system, requiring minimal manipulation.

            View

      • Mesenchymal Stem Cells (MSCs) – Adipose-Derived MSCs (1)

        • Successful Culture of Human Adipose-Derived Mesenchymal Stromal Cells (ASCs) in a Functionally Closed Automated Cell Expansion System

          • This white paper demonstrates that robust ASC ex vivo expansion is possible using the Quantum system to reach clinically relevant cell yields starting from precultured ASCs. The study compared the doubling times of Quantum system-expanded ASCs with ASCs grown in T25 control flasks as well as characterization of ASCs by trilineage differentiation, morphology and phenotype.

            View

      • Fibroblasts (2)

        • Culture of Normal Human Dermal Fibroblast (NHDF) Cells in a Functionally Closed Automated Cell Expansion System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated, scalable expansion method to reproducibly grow NHDFs to yield clinically relevant doses. Additionally, fibronectin and cryoprecipitate are both shown to be feasible coating reagents that can be used to coat the bioreactor prior to expanding NHDFs.

            View

        • Culture of Normal Human Dermal Fibroblast (NHDF) Cells in a Functionally Closed Automated Cell Expansion System

          • This scientific poster demonstrates how the Quantum system provides a functionally closed, automated, scalable expansion method to reproducibly grow NHDFs to yield clinically relevant doses. Additionally, fibronectin and cryoprecipitate are both shown to be feasible coating reagents that can be used to coat the bioreactor prior to expanding NHDFs.

            View

      • Dendritic Cells (1)

        • Maturation of Dendritic Cells from CD14+ Monocytes in an Automated Functionally Closed Hollow-Fiber Bioreactor System

          • Presented at the 2014 International Society for Cellular Therapy (ISCT) Annual Meeting, this poster describes the transformation of CD14+ monocytes into functionally mature DCs using the Quantum system. Also demonstrated, is how the enhanced manufacturing process control of an automated and functionally closed system helps reduce the labor and open events involved in DC maturation.

            View

      • HEK293 (2)

        • Large-Scale Production of Lentiviral Vector in a Closed System Hollow-Fiber Bioreactor

          • This article from the 2015 issue of Molecular Therapy—Methods & Clinical Development, published by the American Society of Gene & Cell Therapy, outlines how researchers developed a method employing a hollow-fiber bioreactor traditionally used for cell expansion to generate a large-scale system method for the manufacturing of clinical-grade lentiviral vectors suitable for the generation of induced pluripotent stem cells (iPSCs). Their results demonstrated the growth, transfection and vector-producing capability of 293T producer cells in this system.

            View

        • Exploring Good Manufacturing Practice (GMP) Production of Lentiviral Vectors

          • This poster describes the successful large-scale production of clinical-grade lentiviral vector using the Quantum Cell Expansion System at the University of California (UC) Davis Institute for Regenerative Cures.

            View

      • T-Cells (1)

        • new WHITE PAPER: Expansion of T-Cells in an Automated, Functionally Closed Hollow-Fiber Bioreactor System

          • This white paper demonstrates the expansion of T-cells in the Quantum® Cell Expansion System. Results from a single stimulation event showed that the Quantum system produced 90- to 500-fold expansion of T-cells starting from an apheresis blood collection in as few as 13 days. The enhanced manufacturing process control of an automated and functionally closed system can help reduce the labor and the number of open events involved in T-cell expansion, which may result in more efficient production and scale-out of cellular production.

            View

    • Publications (40)

      If you are looking for more information about the Quantum system, browse our publications in the form of brochures, white papers, posters, scientific publications and more. We proudly make all our accomplishments available to everyone.

      Show All

      • Brochures (3)

        • Quantum System Brochure

          • As a novel, automated system for growing adherent cells in a closed environment, Terumo BCT's Quantum system is capable of expanding multiple cell types to provide protocol flexibility. The system's unique controls streamline cell culture, deliver reproducible processes and reduce the risk of contamination. This brochure provides a short overview of the system and key features.

            View

        • Quantum Set R17 (RUO) Brochure

          • The Research Use Only (RUO) Cell Expansion Set R17 is designed for use in the Quantum® Cell Expansion System to provide researchers with the benefits of a functionally closed, automated system, including: variable testing, contamination risk reduction and the completion of research on a scalable and reproducible device. This brochure provides a short overview of the RUO cell expansion set R17 and its key features.

            View

        • Quantum System Accessories Brochure

          • The Quantum system bioreactor, media bag, cell inlet bag and waste bag are used for performing automated cell expansion of adherent cells within a functionally closed environment. This brochure provides a short overview of the components needed to grow and harvest cells using the Quantum system.

            View

      • White Papers (9)

        • new WHITE PAPER: Expansion of T-Cells in an Automated, Functionally Closed Hollow-Fiber Bioreactor System

          • This white paper demonstrates the expansion of T-cells in the Quantum® Cell Expansion System. Results from a single stimulation event showed that the Quantum system produced 90- to 500-fold expansion of T-cells starting from an apheresis blood collection in as few as 13 days. The enhanced manufacturing process control of an automated and functionally closed system can help reduce the labor and the number of open events involved in T-cell expansion, which may result in more efficient production and scale-out of cellular production.

            View

        • Automated Expansion of Human Mesenchymal Stem Cells (MSCs) From Whole Bone Marrow and Precultured Cells Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole bone marrow and precultured cells on the Quantum system utilizing fibronectin or cryoprecipitate as an adhesion promoter.

            View

        • Expansion of Human Mesenchymal Stem Cells (MSCs) in the Quantum System Using Human Platelet Lysate (hPL) as a Cell Culture Media Supplement

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs using human platelet lysate as a cell culture media supplement in conjunction with a cryoprecipitate-coated bioreactor.

            View

        • Automated Ex Vivo Expansion of Human Mesenchymal Stem Cells (MSCs) from Pre-Selected Cells Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole unprocessed bone marrow, eliminating the need for density separation of the bone marrow aspirate.

            View

        • Automated Ex Vivo Expansion of Human Mesenchymal Stem Cells (MSCs) from Unprocessed Bone Marrow Using the Quantum System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated and scalable expansion method to generate clinically relevant yields of MSCs from whole unprocessed bone marrow, eliminating the need for density separation of the bone marrow aspirate.

            View

        • Culture of Normal Human Dermal Fibroblast (NHDF) Cells in a Functionally Closed Automated Cell Expansion System

          • This white paper demonstrates how the Quantum system provides a functionally closed, automated, scalable expansion method to reproducibly grow NHDFs to yield clinically relevant doses. Additionally, fibronectin and cryoprecipitate are both shown to be feasible coating reagents that can be used to coat the bioreactor prior to expanding NHDFs.

            View

        • Glucose and Lactate Measurements as a Predictor of the Human Mesenchymal Stem Cell (MSC) Number in the Quantum System

          • This paper describes the use of glucose and lactate measurements for indirect observations of cell expansion within the Quantum system. The results provide a heuristic for determining when the expansion inlet rate of new media should be increased and a means for approximately determining when a desired number of cells can be harvested.

            View

        • Successful Culture of Human Adipose-Derived Mesenchymal Stromal Cells (ASCs) in a Functionally Closed Automated Cell Expansion System

          • This white paper demonstrates that robust ASC ex vivo expansion is possible using the Quantum system to reach clinically relevant cell yields starting from precultured ASCs. The study compared the doubling times of Quantum system-expanded ASCs with ASCs grown in T25 control flasks as well as characterization of ASCs by trilineage differentiation, morphology and phenotype.

            View

        • Shear Stress Conditions in the Quantum System

          • This white paper compares the shear stress experienced by populations of cells in the Quantum system during cell expansion and harvest with the shear stress created in flask environments. The findings show shear stress created in tissue culture flasks during a harvest procedure is significantly higher than the shear stress created in the hollow-fiber bioreactor of the Quantum system during harvest.

            View

      • Scientific Publications (10)

        • new GMP-Compliant Expansion of Clinical-Grade hMSCs Using a Closed Hollow-Fiber Bioreactor

          • Human mesenchymal stromal/stem cells (hMSCs) have emerged as attractive candidates for cell therapy, tissue repair and tissue engineering. This abstract describes a method for good manufacturing practice (GMP)-compliant expansion of hMSCs from bone marrow aspirate using the Quantum® Cell Expansion System. The method includes protocols for preparation of media, setup of the Quantum system, coating of the hollow-fiber bioreactor, as well as the loading, feeding and harvesting of cells.

            View

        • new Process Automation in Manufacturing of Mesenchymal Stromal Cells

          • This article summarizes the labor-intensive nature of current mesenchymal stem cell (MSC) production and increasing demand for devices designed specifically to automate MSC production. As therapeutic processes involving MSCs move from early- to late-stage clinical trials, an increased need is also arising for both scale-up and scale-out manufacturing. The Quantum® Cell Expansion System is a hollow-fiber continuous perfusion device designed specifically for growing multiple adherent cell types and suspension cells in a functionally closed environment.

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        • new Redefining the Role of Blood Establishments as Raw Material Suppliers, Manufacturers and Distributors for New Cell Therapies: The Blood Systems Experience

          • This article discusses the unique skills and expertise, as well as challenges and considerations, of blood establishments that may pursue market activities extending beyond those typically associated with blood components. Such activities include autologous and allogeneic therapies related to regenerative therapies and personalized medicine, donor testing, patient testing, centralized transfusion services, bone marrow processing to support transplants and other related products and services.

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        • Large-Scale Production of Lentiviral Vector in a Closed System Hollow-Fiber Bioreactor

          • This article from the 2015 issue of Molecular Therapy—Methods & Clinical Development, published by the American Society of Gene & Cell Therapy, outlines how researchers developed a method employing a hollow-fiber bioreactor traditionally used for cell expansion to generate a large-scale system method for the manufacturing of clinical-grade lentiviral vectors suitable for the generation of induced pluripotent stem cells (iPSCs). Their results demonstrated the growth, transfection and vector-producing capability of 293T producer cells in this system.

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        • Quality Control During Manufacture of a Stem Cell Therapeutic

          • This article from the March 2012 issue of BioProcess International outlines how three research and development scientists from ReGenesys use extensive quality control (QC) to ensure the identity, quality and safety of their therapeutic stem cell product. The paper compares their QC results between cells expanded in the Quantum system to cells obtained using conventional flask-based culture.

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        • Managing Contamination Risk While Maintaining Quality in Cell-Therapy Manufacturing

          • With an increasing number of cell therapies becoming available for patient use, the need for controlled and consistent manufacturing and delivery of cell products is increasingly important. The Quantum system's closed cell culture process not only offers control and consistency, but may also relieve labor demands.

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        • Efficient Manufacturing of Therapeutic Mesenchymal Stromal Cells (MSCs) With the Use of the Quantum Cell Expansion System

          • This article was authored by Patrick Hanley of Children's National Medical Center (formerly of Baylor College of Medicine) and published in the August 2014 edition of Cytotherapy. It evaluates the Quantum system for the cost-effective, safe and reproducible expansion of large numbers of MSCs from unprocessed bone marrow compared with a flask-based method currently used in clinical trials. The results show that the Quantum system was able to generate the target cell number of 2.0 × 108 cells in an average of nine fewer days and in half the number of passages required during flask-based expansion.

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        • Genetic Stability of Bone Marrow-Derived Human Mesenchymal Stromal Cells in the Quantum System1

          • In this study, published online in the journal Cytotherapy, a rigorous battery of assays was used to determine the genetic stability of BM-hMSCs selected and produced with the Quantum system. hMSC bone marrow aspirates, obtained from three approved donors were expanded in parallel using T225 culture flasks and the Quantum system. The study concluded that Quantum system-produced BM-hMSCs are of comparable quality and demonstrate analogous genetic stability to BM-hMSCs cultured on tissue culture polystyrene substrates.

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        • Tutorial on Automated Bioreactor for Adherent Culturing

          • This tutorial, written by James Beltzer, PhD, and published online by Genetic Engineering & Biotechnology News, explores today’s most promising bioprocessing solutions for the scale-up of adherent cells. Specifically, this tutorial focuses on the Quantum system, which automates many tasks associated with flask-based culture, including cell seeding, reagent addition, feeding and harvest, and its ability to meet the scale-up requirements of cell therapy manufacturing.

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        • GMP-Compliant Isolation and Expansion of Bone Marrow-Derived MSCs in the Closed, Automated, Quantum Cell Expansion System2

          • This paper investigates the Quantum system as an alternative to conventional tissue flasks for clinical-scale mesenchymal stem cell (MSC) isolation and expansion from bone marrow using platelet lysate. The paper concludes that the Quantum system reliably produces a cellular therapeutic dose in a functionally closed system, requiring minimal manipulation.

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      • Posters (12)

        • Human Mesenchymal Stem Cells (MSC) Genetic Stability in the Quantum Cell Expansion System

          • This poster explains the assays and results used by Terumo BCT to determine the genetic stability of MSCs produced in the Quantum system. The results conclude that the Quantum system produces cell quality and genetic stability comparable to traditional flasks and meets the Stem Cell Transplantation International Society of Cell Therapy Guidelines for MSC phenotype.

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        • Using Multiple Freeze-Thawed Cryoprecipitate (CPPT) as a Coating Reagent

          • The results of this study presented at the International Society for Stem Cell Research (ISSCR) in 2013 show that CPPT that has undergone two freeze-thaw events can be used to coat the Quantum system hollow-fiber membrane and produce cell yields that are comparable to CPPT that was only thawed once. These results were demonstrated using: bone marrow-derived mesenchymal stem cells, adipose-derived mesenchymal stem cells and normal human dermal fibroblasts. The use of multiple freeze-thawed CPPT coating offers a more cost-effective coating mechanism.

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        • Expansion of Adult Bone Marrow-Derived Mesenchymal Stem Cells (BMMSCs) in the Quantum System Under Hypoxic and Normoxic Conditions

          • The results of this study presented at the International Society for Stem Cell Research (ISSCR) in 2013 demonstrate that clinical-scale yields of MSCs may be expanded on the Quantum system under both normoxic and hypoxic conditions. There was no significant difference in cell yield, viability, phenotypic, morphological or differentiation potential observed between cells cultured under either condition.

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        • Process Optimization for Expansion of Bone Marrow-Derived Mesenchymal Stem Cells (MSCs)

          • This poster, presented at the International Society for Cellular Therapy (ISCT) in 2011, illustrates the diverse functionality and process control of the Quantum system. An optimized process has many benefits including limiting the media used, thus lowering costs, increasing the cell yield and creating a more uniform cell harvest.

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        • Quantitative Tri-Lineage Differentiation Assays for Mesenchymal Stem Cells (MSCs) Grown in the Quantum Cell Expansion System

          • This poster, presented at the International Society for Cellular Therapy (ISCT) in 2011, reports on quantitative assays for MSC adipocyte, osteocyte and chondrocyte differentiation for MSCs grown in the Quantum system. These assays address an unmet need in the MSC field for quantitative differentiation assays eliminating user-to-user subjective assessments and, more importantly, allowing for lab-to-lab quantitative comparisons.

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        • Manufacture of Bone Marrow Stromal Cells (BMSCs) Using a Novel Closed System Maintains Cell Characteristics Consistent With Flask-Based Methods

          • This poster, authored by the NIH, describes three major findings. First, cell yield was in all test cases higher when cells were grown in the Quantum system compared to Cell Factories. Second, BMSCs grown in the Quantum system have similar phenotypes compared to BMSCs grown in Cell Factories. Lastly, BMSCs from both the Quantum system and Cell Factories are able to differentiate into adipocytes. In conclusion, growth in the Quantum system does not alter the differentiation potential of the subset of stem cells within the BMSC population.

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        • Maturation of Dendritic Cells from CD14+ Monocytes in an Automated Functionally Closed Hollow-Fiber Bioreactor System

          • Presented at the 2014 International Society for Cellular Therapy (ISCT) Annual Meeting, this poster describes the transformation of CD14+ monocytes into functionally mature DCs using the Quantum system. Also demonstrated, is how the enhanced manufacturing process control of an automated and functionally closed system helps reduce the labor and open events involved in DC maturation.

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        • Exploring Good Manufacturing Practice (GMP) Production of Lentiviral Vectors

          • This poster describes the successful large-scale production of clinical-grade lentiviral vector using the Quantum Cell Expansion System at the University of California (UC) Davis Institute for Regenerative Cures.

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        • Expanding Human Mesenchymal Stem Cells (MSCs) to Therapeutic Doses Using Human Platelet Lysate (hPL) in the Quantum Cell Expansion System

          • The aim of this study was to evaluate the flexibility of the Quantum system when users require different combinations of adherence promoters and media supplements. The results demonstrate that the Quantum system can be used effectively to culture MSCs using exclusively human-derived reagents.

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        • CD146 Expression as a Biomarker for hMSC Potency in Normal Cells Expanded With the Quantum System

          • The Terumo BCT Innovation and Development (I&D) team has identified the CD146 surface biomarker expression by flow cytometry as an early indicator of hMSC differentiation potency at the time of harvest from the Quantum system. A poster has been developed to illustrate the study and its findings.

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        • Culture of Normal Human Dermal Fibroblast (NHDF) Cells in a Functionally Closed Automated Cell Expansion System

          • This scientific poster demonstrates how the Quantum system provides a functionally closed, automated, scalable expansion method to reproducibly grow NHDFs to yield clinically relevant doses. Additionally, fibronectin and cryoprecipitate are both shown to be feasible coating reagents that can be used to coat the bioreactor prior to expanding NHDFs.

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        • Shear Stress Conditions in the Quantum Cell Expansion System

          • This scientific poster compares the shear stress experienced by populations of cells in the Quantum system during cell expansion and harvest with the shear stress created in flask environments. The findings show shear stress created in tissue culture flasks during a harvest procedure is significantly higher than the shear stress created in the hollow-fiber bioreactor of the Quantum system during harvest.

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      • Case Studies (2)

        • newScaling Cell Therapy Manufacturing

          • How Quantum® Cell Expansion Systems increased cell production quantities from one billion cells to commercially relevant batch sizes. Specifically, this case study outlines how the efficient use of both human and facility resources maintained a cost savings of 40 percent relative to manual manufacturing methods.

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        • The Quantum Cell Expansion System’s Valuable Role in Cell Production Requirements for ReGenesys

          • This case study outlines the benefits experienced at ReGenesys by using the Quantum system. Specifically this case study outlines a ten-fold reduction in labor and 40 percent cost savings.

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      • News (6)

        • Innovative Device Company Products Seen Rapidly Reaching Exhibit Floor

          • This article from the 2012 American Society of Hematology meeting features an interview where Dr. Patrick Hanley, PhD, of Baylor College of Medicine describing the benefits he has seen with the Quantum system using MSCs. This novel approach requires about 20 percent of the staff hours involved in the preparation, and only 0.2 percent of the human contact needed with the manual technique.

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        • Terumo BCT Announces Collaboration to Evaluate Clinical Cell Production Using the Quantum System

          • This 7 August 2014 press release announces that the Quantum system is now under evaluation by Athersys, Inc. as a manufacturing platform for Athersys' allogeneic multipotent adult progenitor cell (MAPC®) products. ReGenesys, Athersys’ affiliate in Belgium, will evaluate the potential for scaling their cell culture process by using multiple units to meet its manufacturing requirements and will further quantify advantages over standard cell manufacturing approaches.

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        • Turkey’s Baskent University Hospital Manufactures MSCs Using Quantum System

          • This Turkish language article shares the experience of Ilknur Kozanoglu, Director of the Cell Processing Unit at Baskent University Hospital in Adana, Turkey, who was able to manufacture mesenchymal stem cells (MSCs) to a clinical scale yield using the closed, automated Quantum system.

            View

        • First Bone Marrow Expansion at Pisa Hospital Uses Quantum System

          • This Italian language article discusses Dr. Fabrizio Unleash’s work with the unit of Transfusion Medicine and Transplantation Biology at Pisa Hospital, and Italy’s first bone marrow expansion using the Quantum system.

            View

        • Athersys Receives Cell Therapy Manufacturing Award

          • This Athersys, Inc. press release announces that its subsidiary, ReGenesys BVBA, has received the BioProcess International Award for the “Technical Application of the Decade” in Manufacturing for their work in demonstrating that MultiStem cell therapy can be successfully manufactured on a large scale, using an automated bioreactor system.

            View

        • Blood Systems, BSRI, the University of California San Francisco (UCSF), Terumo BCT, and the Department of Defense Form Collaborative Group to Investigate New Area of Cell Therapy

          • This announcement, by Blood Systems Laboratories, discusses the formation of a collaborative group, including Blood Systems, BSRI, the University of California San Francisco (UCSF), Terumo BCT, and the Department of Defense. The group will investigate whether Mesenchymal stem cell (MSC) transplantation can regenerate healthy tissue and restore damaged tissue in trauma patients.

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    • Customer Testimonials/Press (9)

      View our proud customers and learn what they have to say about the Quantum system.

        • newThis Angiocrine Biosciences Inc. press release from January 2016 shares the company’s work to scale up cell manufacturing with Terumo BCT and the Quantum® Cell Expansion System

          • Through this collaboration, Angiocrine is advancing its preclinical therapeutic programs in a predictable and effective manner, bringing novel cell therapy solutions closer to commercialization.

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        • Terumo BCT Quantum System is Shown Cost-Effective for Scaled Cell Manufacturing

          • This press release, is an evaluation in producing MAPCs® showed 40 percent cost savings combined with meeting cell quality needs

            View

        • Innovative Device Company Products Seen Rapidly Reaching Exhibit Floor

          • This article from the 2012 American Society of Hematology meeting features an interview where Dr. Patrick Hanley, PhD, of Baylor College of Medicine describes the benefits he has seen with the Quantum system using MSCs. This novel approach requires about 20 percent of the staff hours involved in the preparation, and only 0.2 percent of the human contact needed with the manual technique.

            View

        • Quality Control During Manufacture of a Stem Cell Therapeutic

          • This article from the March 2012 issue of BioProcess International outlines how three research and development scientists from ReGenesys use extensive quality control (QC) to ensure the identity, quality and safety of their therapeutic stem cell product. The paper compares their QC results between cells expanded in the Quantum system to cells obtained using conventional flask-based culture.

            View

        • The Quantum Cell Expansion System’s Valuable Role in Cell Production Requirements for ReGenesys

          • This case study outlines the benefits experienced at ReGenesys by using the Quantum system. Specifically this case study outlines a ten-fold reduction in labor and 40 percent cost savings.

            View

        • Terumo BCT Announces Collaboration to Evaluate Clinical Cell Production Using the Quantum System

          • This 7 August 2014 press release announces that the Quantum system is now under evaluation by Athersys, Inc. as a manufacturing platform for Athersys' allogeneic multipotent adult progenitor cell (MAPC®) products. ReGenesys, Athersys’ affiliate in Belgium, will evaluate the potential for scaling their cell culture process by using multiple units to meet its manufacturing requirements and will further quantify advantages over standard cell manufacturing approaches.

            View

        • Turkey’s Baskent University Hospital Manufactures MSCs Using Quantum System

          • This Turkish language article shares the experience of Ilknur Kozanoglu, Director of the Cell Processing Unit at Baskent University Hospital in Adana, Turkey, who was able to manufacture mesenchymal stem cells (MSCs) to a clinical scale yield using the closed, automated Quantum system.

            View

        • First Bone Marrow Expansion at Pisa Hospital Uses Quantum System

          • This Italian language article discusses Dr. Fabrizio Unleash’s work with the unit of Transfusion Medicine and Transplantation Biology at Pisa Hospital, and Italy’s first bone marrow expansion using the Quantum system.

            View

        • Athersys Receives Cell Therapy Manufacturing Award

          • This Athersys, Inc. press release announces that its subsidiary, ReGenesys BVBA, has received the BioProcess International Award for the “Technical Application of the Decade” in Manufacturing for their work in demonstrating that MultiStem cell therapy can be successfully manufactured on a large scale, using an automated bioreactor system.

            View

        • Blood Systems, BSRI, the University of California San Francisco (UCSF), Terumo BCT, and the Department of Defense Form Collaborative Group to Investigate New Area of Cell Therapy

          • This announcement, by Blood Systems Laboratories, discusses the formation of a collaborative group, including Blood Systems, BSRI, the University of California San Francisco (UCSF), Terumo BCT, and the Department of Defense. The group will investigate whether Mesenchymal stem cell (MSC) transplantation can regenerate healthy tissue and restore damaged tissue in trauma patients.

            View

Learn More

 Overview
 How It Works
 Manual versus Automated Comparison Video
 Research



*Customers are responsible for validating the use of the Quantum system to expand cells for their intended therapy.​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​

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The Quantum system can be used in the production process of the following therapies:*

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