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COBE® 2991 Protocols
Thanks to its flexible diaphragm, the COBE 2991 Cell Processor has the ability to express fluids and/or cells for removal or collection during centrifugation.
Red Blood Cell (RBC) Washing
Deglycerolized Frozen RBCs
Preparation of Leukocyte Concentrates
Collection of Young RBCs (Neocytes)
Bone Marrow Processing Using a Density Gradient Separation Medium
Bone Marrow Concentration
Mononuclear Cell (MNC) Washing
Cell Concentration
Large Volume Wash (Shed Blood Salvage)
RBC Washing

This protocol removes unwanted plasma and plasma proteins, metabolic waste products, microaggregates and platelets from a packed RBC unit using processing/washing solution.

  • Packed RBC protocols end after the Supernatant-Out operation, leaving the ending cell product more concentrated
  • Resuspended RBC protocols end after the Agitate/Wash-In operation, leaving the ending cell product resuspended in processing/washing solution

Use the COBE 2991 Cell Processing Set

Capabilities
  • Average removal of 84.7 percent of white blood cells (WBCs) from packed RBCs using a 1 L wash with two 3-second RBC overrides1
  • Average recovery of 85.5 percent of RBCs from packed RBCs using a 1 L wash with two 3-second RBC overrides1
  • Average removal of 99.4 percent of supernatant protein from packed RBCs using a 1 L wash with two 3-second RBC overrides1
  • Average removal of 93.3 percent of WBCs from packed RBCs using a 1 L wash with two 4-second RBC overrides2
  • Average recovery of 82.1 percent of RBCs from packed RBCs using a 1 L wash with two 4-second RBC overrides2
Benefits
  • Reduces non-hemolytic febrile reactions
  • Reduces anaphylactic reactions
  • Reduces urticarial reactions
Deglycerolized Frozen RBC Units

These protocols remove glycerol from thawed RBC units using decreasing concentrations of processing/washing solutions.

The following protocols end after the Supernatant-Out operation, leaving the ending cell product more concentrated:

  • Deglycerolized High-Glycerol Frozen RBCs
  • Deglycerolized Low-Glycerol Frozen RBCs
  • Deglycerolized High-Glycerol Frozen RBCs Low Volume Unit (60 mL to 80 mL)

The Deglycerolized Resuspended High-Glycerol Frozen RBCs protocol ends after the Agitate/Wash-In operation, leaving the ending cell product resuspended in processing/washing solution.

Use with these disposable sets:
  • COBE 2991 Cell Processing Set
  • COBE 2991 Double Coupler Adapter (if required)
Capabilities
  • Average removal of 95 percent of the WBCs from RBCs frozen using the high-glycerol method using 2.2 L of sodium chloride solution during the deglycerolization and washes3
  • Average removal of 95 percent of platelets from RBCs frozen using the high-glycerol method using 2.2 L of sodium chloride solutions during the deglycerolization and washes3
  • Average removal of 89 percent of microaggregates ranging from 5.6 µm to 40 µm in diameter from RBC units frozen using the high-glycerol method4
Benefits
  • Reduces transfusion reactions
  • Reduces anaphylactic reactions
  • Reduces urticarial reactions
Preparation of Leukocyte Concentrates

This protocol can be used to prepare a leukocyte concentrate from a single unit of non-leukoreduced fresh blood.

  • Utilizes differential sedimentation of blood components according to their specific gravities within a gravitational field
  • Accelerates the sedimentation process, allowing the relative isolation of the granulocyte-rich fraction, which may then be expressed into a transfer pack for subsequent use

Use with the COBE 2991 Cell Processing Set

Collection of Young RBCs (Neocytes)
  • Utilizes differential sedimentation of blood components according to their specific gravities within a gravitational field
  • Accelerates the sedimentation process, allowing the relative isolation of the young RBC-rich fraction, which may then be expressed into a transfer pack for subsequent use
  • Allows for the isolation and collection of the younger half of the original unit

Use with the COBE 2991 Cell Processing Set

Bone Marrow Processing Using a Density Gradient Separation Medium
  • The goal of in vitro manipulation of bone marrow is to reduce the volume and RBC contamination of the bone marrow harvest while minimizing the loss of MNCs, in particular stem and progenitor cells
  • The triple processing set reduces the processing time and the need for multiple single processing sets
Use with the following disposable sets:
  • COBE 2991 Triple Processing Set
  • COBE 2991 Double Coupler Adapter
  • COBE 2991 Female Luer Connector
Capabilities
  • Semi-automated processing and purification
  • Volume reduction
  • RBC, polymorphonuclear cell and platelet reduction
  • Density gradient separation possible
Benefits
  • Reduces procedure time and cost
  • Reduces cell clumping
  • Reduces RBC stroma and hemoglobin
  • Reduces dimethylsulfoxide volume from previously frozen product
  • Reduces the number of ABO-incompatible RBCs from marrow prior to transplant
Bone Marrow Concentration
  • Prepares a concentrated buffy coat from a large volume of bone marrow; bone marrow concentration is based upon the principle of differential sedimentation of blood components according to their specific gravities within a gravitational field
  • Accelerates the sedimentation process, allowing the relative isolation of the buffy coat, which may then be expressed into a transfer pack for subsequent processing or use
Use with the following disposable sets:
  • COBE 2991 Cell Processing Set
  • COBE 2991 Double Coupler Adapter (if required)
MNC Washing
  • Removes cryopreservative, hemolyzed RBCs or other contaminants from MNC products
Use with the following disposable sets:
  • COBE 2991 Cell Processing Set
  • COBE 2991 Double Coupler Adapter (if required)
Cell Concentration
  • Concentrates expanded cells from large volumes
Use with the following disposable sets:
  • COBE 2991 Cell Processing Set
  • COBE 2991 Double Coupler Adapter
Large Volume Wash (Shed Blood Salvage)
  • Washes a large volume of salvaged shed blood for autotransfusion or source blood for industrial/commercial use
Use with the COBE 2991 Cell Processing Set
1Wooten MJ, "Use and Analysis of Saline Washed Red Blood Cells." Transfusion 1976; 16 (5): 464–468.
2Bryant LR, et al., "Optimal Leukocyte Removal from Refrigerated Blood With the IBM 2991 Blood Cell Processor." Transfusion 1978; 18 (4): 469–471.
3Crowley JP, et al., "The Purification of Red Cells for Transfusion by Freeze-Preservation and Washing Versus Red Cell Recovery and Residual Leukocytes After Freeze-Preservation With High Concentrations of Glycerol and Washing in Various Systems." Transfusion 1977; 17 (1): 1–7.
4Telischi M, et al., "Microaggregate Counts in Frozen-Preserved Erythrocytes: Effects of Washing in Three Blood Processors and Filtration." Blood 1977; 50 (4): 743–748.

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